摘要
本研究应用双功能偶联剂小分子间苯二甲基二异氰酸盐 (m xylylen diisocyanate ,简称XDI)作桥梁 ,将短肽RGD(GLY ARG GLY ASP SER PRO LYS)粘附生长因子偶联到卵清白蛋白 (Ovalbumin ,简称OVA)载体上 ,对偶联物RGD OVA经SDS 聚丙烯酰胺凝胶纯化和紫外光谱测定 ,确定RGD与OVA偶联比为 11∶1,用偶联物对兔子进行背部小剂量多点免疫注射 ,诱发出抗RGD抗体 ,利用得到的兔抗RGD抗体IgG包埋于丝素材料内 ,并借助抗原抗体的结合力 ,结合RGD在该丝素膜表面 ,对这种结合有RGD抗体的丝素膜进行培养血管内皮细胞(Endothelialcell,简称EC)的试验 ,结果表明 ,结合有RGD的丝素膜与其对照组相比 ,细胞数量有显著提高。
This study aimed at the biological modifications of silk membranes to create a novel biomaterial in order to improve membrane compatibility and promote its clinical application, especially in cardiovascular grafting. The adhesive and growth protein, Arg-Gly-Asp(RGD) plays an important role in cell adhesion, migration and growth. The study tried to bury the immunoprotein IgG of RGD in silk solution, yielding a fibroin membrane binding RGD through IgG affinity. As RGD is a hapten, the study used the ovalbumin as the carrier and conjugated RGD to the ovalbumin, it was proved that the cptimal binding ratio was 11 to 1. It was dermonstrated that the RGD bound silk membrane stimulated growth of the endothelial cells and promoted tissue regeneration in the silk scaffold.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2003年第6期537-542,共6页
Chinese Journal of Biomedical Engineering
基金
国家重点基础研究发展规划资助项目 (G19990 64 7)
关键词
RGD
抗体
半抗原
丝素膜
血管内皮细胞
Adhesion
Antibodies
Biological membranes
Biomaterials
Cardiovascular system
Cells
Growth kinetics
Proteins
Silk
