摘要
目的 评价微载体粘附培养的肝细胞腹腔内移植对D 氨基半乳糖盐酸盐诱导的大鼠急性肝功能衰竭的治疗作用。方法 胶原酶灌注分离大鼠肝细胞 ,行Cytodex 3粘附培养 ,移植 1× 10 7个微载体粘附肝细胞至D 氨基半乳糖盐酸盐诱导的急性肝功能衰竭大鼠腹腔内 ,比较各组间生存率、肝功能及肝脏病理改变情况以评估疗效。结果 微载体移植组大鼠存活率明显高于空载体组 ,移植 5d后两组比较差异显著 (P <0 .0 5 ) ;空载体组大鼠肝功能及肝脏修复明显迟于微载体组。结论 微载体粘附培养的肝细胞腹腔内移植可对急性肝衰竭大鼠提供代谢支持作用 ,提高急性肝衰竭大鼠存活率。
Objective To evaluate the effects of intraperitoneal transplantation of microcarrier-attached hepatocytes on acute liver failure(ALF)in rats induced by D-galactosamine(D-GalN). Methods Rat hepatocytes were isolated by collagenase perfusion, and cultured in Cytodex 3.The microcarrier-attached rat hepatocytes(1×10 7 cells) were intrapertioneally transplanted into rats with 2.0 g/kg D-GalN induced(24 hours) ALF. The effects of transplantation of microcarrier-attached hepatocytes were evaluated by comparing liver function and hepatic pathologic changes as well as survival rates throughout the entire experimental period of the three groups.Results The survival rate of the rats in the group which received microcarrier cultured hepatocytes was much higher than that of vacant microcarriers group. Differences of survival rates between microcarriers group and vacant microcarriers group were significant on the 5th day of transplantation and afterwards(P<0.05).The improvement of liver function and liver histologic damage in the survived rats of nude microcarriers group was slower than rats in microcarriers group.Conclusion Transplanted microcarrier-attachecd hepatocytes provide sufficient metabolic support to the damaged recipient liver and increase the survival rates of ALF rats.
出处
《肝脏》
2003年第4期27-30,共4页
Chinese Hepatology
