摘要
本文通过聚丙烯酰胺凝胶电泳(SDS—PAGE)法,分析了蓖麻蚕卵黄蛋白质组分,对卵黄磷蛋白(VitellinVn,)进行了鉴定、纯化,并制备了抗血清。对卵黄磷蛋白的分子量及相对含量进行了测定。并证明卵黄磷蛋白若处在酸性pH条件下能降解。
The coniposition of the yolk proteins was analyLed by the method of SDS PAGE. The vitellin was identified and purified,and the antiserum to the vitellin wasprepared;Ttie niolecular weight of the vItellin was determined。Meanwhile,the relativequantity of the vitellin was calculated.The vitellin was also found to be hydrolyzed at aciticpH condition。
出处
《华东昆虫学报》
1995年第1期81-84,共4页
Entomological Journal of East China
基金
山东大学青年科学基金
国家教委留学回国人员基金
关键词
蓖麻蚕
卵黄磷蛋白
鉴定
纯化
抗血清制备
Philosamia cynthia ricina,vitellin, purification, antiserum.