摘要
叙述了α核素^(211)At通过对苯二胺或联苯胺重氮盐标记癌胚抗原单克隆抗体(CEA-McAb)的方法。将芳香二胺转成重氮盐,随后与^(211)At和CEA-McAb反应,生成^(211)At-CEA-McAb偶联物。反应与纯化大约需要2.5h,Sephadex G-75用于从反应产物中分离标记单克隆抗体(McAb),产率不低于初始^(211)At放射性活度的30%。^(211)At-CEA-McAb的比活度可达(2.2—3.7)×10~4Bq/)μg(McAb)。小鼠体内组织的分布实验结果表明,制得的^(211)At-CEA-McAb偶联物在体内条件下稳定。
A method was described for labelling CEA monoelonal antibody (CEA-McAb) with the a-emitting nuclide ^(211)At via diazo salts of p-phenylenediamine or benzidine. Aromatic diamines were transformed into diazo salts and subsequently both ^(211)At and CEA-McAb react with diazo salts to produce ^(211)At-CEA-McAb conjugates. The reaction and purification required about 2.5 h. Sephadex G-75 column was used to separate the labelled CEA-McAb from reactive products and the labelling yield was at least 30% of the initial activity of ^(211)At. The specific activity of ^(211)At-CEA-McAb (2.2—3.7)×10~4Bq/ug(McAb) could be achieved. The results of tissue distribution of ^(211)At in mice showed that ^(211)At-CEA-McAb conjugates were stable in vivo.
出处
《同位素》
CAS
北大核心
1992年第2期65-71,共7页
Journal of Isotopes
基金
国家自然科学基金
