摘要
从 30 0条RAPD随机引物中筛选出扩增多态性丰富的 13条引物 ,对寄主来源不同的 2 9个寄生疫霉 (Phy tophthoraparasitica)菌株进行基因组DNA遗传变异分析。用筛选出的 13条引物对供试菌株进行RAPD PCR扩增 ,共产生 139条RAPD条带 ,其中 133条为多态性条带 ,多态检测率为 95 .7%。利用PopGeneVersion 1.31软件对供试菌株间的遗传距离进行聚类分析并构建系统树状图 ,供试 2 9个菌株被划分为 5个遗传聚类组 ,不同菌株间具有丰富的遗传变异。其中寄主为烟草 (Nicotianaspp .)、腊梅 (Chimonanthuspraecox)、凤尾兰 (Yuccagloriosa)和西番莲 (Passifloraedulis)的菌株的遗传结构与其寄主来源具有明显的相关性 ,而寄主为刺槐 (Sophorachinensis)和番茄 (Lycopersicumesculentum)的菌株与其寄主来源的相关性较小 ,来源于不同寄主植物的菌株之间遗传距离较远。结果提示在寄生疫霉与其不同寄主植物的长期协同进化过程中 。
Genomic fingerprints of 29 isolates of the pathogen Phytophthora parasitica from different hosts were analyzed by using 13 RAPD primers selected from 300 random primers with abundant polymorphisms. Genomic DNA from different isolates were amplified with the 13 selected primers and 139 marked bands were produced, of which 133 bands were polymorphic, accounting for 95.7% of the total bands produced. Based on the genetic distance, a dendrogram was constructed with the software PopGene Version 1.31, and the 29 tested isolates were divided into five genetic groups. Analysis showed that the genetic variation was very rich among the tested isolates. The isolates from Nicotana tabaccum (tobacco), N. glutinosa (tobacco), Chimonanthus praecox (wintersweet), Yucca spp. (beargrass) and Passiflora edulis (passionflower) were clustered into one group, and showed high relativity with their hosts. The similarity of Lycopersicum esculentum (tomato) isolates was about 60.9%, and they could be clustered into two distinct groups. The similarity of the five isolates from Sophora chinensis (sophora) was about 66.7%～ 88.4%, and they were also clustered into two groups when the dendrogram was constructed at the value point of 0.24 of the genetic distance. The results indicate that the host plants could have some effect on the genomic structure of the pathogen during the co evolution between pathogen and their hosts.
出处
《生物多样性》
CAS
CSCD
2003年第6期486-490,共5页
Biodiversity Science
基金
国家自然科学基金资助项目 (3 0 2 70 865 )
教育部跨世纪优秀人才基金资助项目
