摘要
AIM:To investigate the effect of complex amino acid imbalance on the growth of tumor in tumor-bearing(TB)rats.METHODS:Sprague-Dawlley(SD)rats underwent jejunostomy for nutritional support.A suspension of Walker256 carcinosarcoma cells was subcutaneously inoculated.TB rats were randomly divided into groups A,B,C and D according to the formula of amino acids in enteral nutritional solutions,respectively.TB rats received jejunal feedings supplemented with balanced amino acids(group A),methionine-depleted amino acids(group B),valine-depleted amino acids(group C)and methionine-and valine-depleted complex amino acid imbalance(group D)for 10 days.Tumor volume,inhibitory rates of tumor,cell cycle and life span of TB rats were investigated.RESULTS:The G_(0)/G_(1)ratio of tumor cells in group D(80.5±9.0)%was higher than that in groups A,B and C which was 67.0±5.1%,78.9±8.5%,69.2±6.2%,respectively(P<0.05).The ratio of S/G_(2)M and PI in group D were lower than those in groups A,B and C.The inhibitory rate of tumor in groups B,C and D was 37.2%,33.3%and 43.9%,respectively(P<0.05).The life span of TB rats in group D was significantly longer than that in groups B,C,and A.CONCLUSION:Methionine/valine-depleted amino acid imbalance can inhibit tumor growth.Complex amino acids of methionine and valine depleted imbalance have stronger inhibitory effects on tumor growth.
基金
Hubei Provincial Health Bureau,No.W98016
the Education Committee of Hubei Province,No.2001A 14005
