摘要
目的 建立一套Y染色体微缺失的多重PCR筛查方法。方法 建立5套稳定和可靠的多重PCR筛查方法,对进行ICSI治疗的26例无精子症患者和进行睾丸活检的30例无精子症患者进行Y染色体微缺失的检测。结果 在56例无精子症患者中发现5例AZFc/DAZ缺失,2例同时具有AZFc/DAZ和AZFb/RBM1的缺失,1例仅具有sY153一个片段的缺失。结论 本研究Y染色体微缺失的多重PCR筛查方法是易行和可靠的,对无精子症患者有必要进行Y染色体微缺失的常规筛查。
Objective To develop a multiplex PCR protocol for routine screening of microdeletions on the Y chromosome. Methods Five multiplex sets were established. 26 azoospermic men undergoing ICSI and 30 azoospermic men undergoing testicular biopsy were screened for microdeletions of Y chromosome. Results In 56 azoospermic men, it was founded 5 patients with AZFc/DAZ microdeletions, 2 patients with AZFc/DAZ and AZFb/RBMl microdeletions, and 1 patient with single sY153 microde-letion. Conclusion The multiplex PCR protocol presented in this study is an easy and reliable method for detection of microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic patients is essential.
出处
《中国男科学杂志》
CAS
CSCD
2003年第6期369-372,共4页
Chinese Journal of Andrology
