摘要
目的 :观察兴奋性神经递质精氨酸对于可溶性淀粉样前体蛋白 (s APP)释放的影响。方法 :雄性 SD大鼠断头取海马后于 0～ 4 o C Krebs- Ringer液中快速振动切片 ,切片于 37o C预孵育后置含精氨酸或不含精氨酸 Krebs- Ringer液(对照 )中孵育后于 15 0 0 0 g离心取上清液 ,应用二辛可宁酸法测定上清液总蛋白含量 ;释放入孵育液中的 s APP以Western blot方法测定。结果 :正常孵育的海马脑片能够分泌基础量的 s APP,精氨酸刺激能够导致海马脑片释放 s APP增加 ,但是没有导致新蛋白的产生。结论 :精氨酸在 10 - 9～ 10 μmol/ L浓度范围内 ,均能够导致海马脑片分泌 s
Object: To observe the effects of excitatory neurotransmitter L-arginine on the release of the soluble amyloid precursor protein (sAPP). Methods: Male Sprague-Dawley rats were decapitated and hippocampus were dissected at 4℃ , then hippocampal slices were quickly prepared in the medium of Krebs-Ringer buffer at 0~4℃ by a vibration chopper. After the slices were preincubated in Krebs-Ringer buffer at 37℃, they were stimulated with various concentrations of L-arginine in Krebs-Ringer buffer. The supernatant were removed from the solution after centrifugation at 15 000 g. Contents of total protein were measured by bicinchoninic acid assay (BCA). The sAPP derivates released into the incubation medium were assayed by Western blot analysis. Results: L-arginine has increased the release of the soluble amyloid precursor protein (sAPP) in incubated hippocampal brain slices. However, failed to produce special protein. Conclusion: L-arginine has positive effects on the release of the soluble amyloid precursor protein (sAPP) from concentration of 10 -15 mol/L to 10 -5 mol/L.
出处
《天津药学》
2003年第6期1-4,共4页
Tianjin Pharmacy
基金
国家自然科学基金资助项目 (No.39770 80 7)
