摘要
目的 了解北京天坛医院和北京朝阳医院产超广谱 β -内酰胺酶 (ESBLs)菌株的耐药情况 ,并对携带的ESBLs基因型进行调查。方法 对 5 2株大肠埃希菌、肺炎克雷伯菌进行琼脂稀释法、等电点测定和聚合酶链反应克隆测序。结果 产ESBLs菌株对多种抗生素耐药 ;克隆测序分别为CTX -M -14、2 2、2 4和SHV -2、12型。结论 密切监控ESBLs菌株的产生 ,追踪调查其基因型 。
Objective To investigate drug-resistance and the genotypes of extended-spectrum β lactamases (ESBLs) producers in Beijing Tiantan Hospital and Beijing Chaoyang Hospital. Methods Standard agar dilution method, transconjugation test, isoelectric focusing, DNA sequencing were preformed in 52 clinical isolates of Escherichia coli and Klebsiella pneumoniae. Results ESBLs producers were resistant to many antibiotics. DNA sequencing of amplicons of 52 strains revealed that ESBLs-encoded genes were CTX-M-14, 22,24 and SHV-2,12,respectively. Conclusions We should pay great attention to detect ESBLs producers and investigate their genotypes, so that we could prevent regional prevalence of ESBLs producers and utilize antibiotics reasonably.
出处
《中国医师杂志》
CAS
2004年第1期13-15,共3页
Journal of Chinese Physician
