摘要
的 :检测并观察乙型肝炎患者血清标志物(HBsAg、HBsAb、HBeAg、HBeAb、HBcAb)、前S1(Pre_S1)蛋白和HBV_DNA的相关性 ,探讨新型指标Pre_S1检测的临床意义。方法 :(1)用ELISA法测定100例乙型肝炎患者及35例健康体检者的乙肝血清标志物 ,并按结果分为3组。(2)各组用乙肝病毒Pre_S1蛋白诊断试剂检测Pre_S1蛋白。(3)用定量PCR检测上述标本的HBV_DNA。(4)观察检测血清标志物 ,Pre_S1和HBV_DNA相关性。结果 :Pre_S1蛋白在HBeAg( +)组中检出率 (82.50% )和HBeAg( -)组中检出率 (46.67 % )差别有统计学意义 ;Pre_S1蛋白与HBV_DNA有良好相关性。结论 :3项实验指标之间具有良好相关性 ,Pre_S1蛋白是HBV感染与复制的新指标 ,有望作为乙型肝炎新的检测手段和新的标志物。
Objective:To study the relationship between serological markers(HBsAg,HBsAb,HBeAg,HBeAb,HBcAb),pre_S1protein and HBV_DNA in patients with hepatitis B.Methods:(1)ELISA was used to measure the serological markers in patients with hepatitis B and normal persons.According to the results,they were divided into three groups.(2)Their pre-S1protein was measured with HBV pre-S1diagnosis reagents.(3)The HBV-DNA was detected with quantified-PCR.(4)The relationship between serological markers,pre-S1protein and HBV-DNA was observed.Results:The detectable rate of pre-S1protein in positive HBeAg group(82.50%)was higher than that in negative HBeAg group(46.67%)(P<0.01).Conclusions:There was a sig-nificant relationship between serological markers,pre_S1protein and HBV_DNA.The pre_S1protein would be used as a new marker for infection and replication of HBV.
出处
《天津医药》
CAS
北大核心
2004年第1期19-21,共3页
Tianjin Medical Journal
