摘要
目的 探讨视黄酸 (RA)对人心、脑特异表达基因NDRG4在人中枢神经元前体细胞NT2中表达的影响。 方法 培养回收分别经RA(10 μmol L)处理 1、2周及未经处理的NT2细胞 ,提取总RNA进行NDRG4表达的Northern印迹分析 ,并应用NDRG4变异体NDRG4 B及NDRG4 H5’ 端特异序列寡核苷酸引物对NT2细胞总RNA进行逆转录聚合酶链反应 (RT PCR)。 结果 Northern印迹分析表明 ,RA刺激可使NT2细胞NDRG4表达显著增加。RT PCR结果表明 ,NDRG4 H及NDRG4 B变异体mRNA表达对RA反应不同 ,受RA刺激后前者表达逐渐上调 ,后者表达则无明显变化。 结论 NDRG4可能与中枢神经元发育及生长有关。对NDRG4基因的深入研究将为了解其在阿尔茨海默病 (AD)等神经元变性疾病发病机制中的作用提供某些线索。
Objective To investigate the effect of retinoic acid(RA) on the expression of NDRG4, a gene specifically expressed in human brain and heart, in human neuronal precursor cell line NT2. Methods NT2 cells were cultured and recovered before and 1 or 2 weeks after being treated with retinoic acid. Total RNA was prepared and subjected to Northern blot analysis. Reverse transcription-polymerase chain reaction(RT-PCR) was also performed using the primers spanning the 5'-region specific to NDRG4-H or NDRG4-B variant cDNAs, respectively, and the primer spanning the common region shared by both variant cDNAs. Results The expression of NDRG4 was significantly higher in NT2 cells treated by RA than in NT2 untreated cells. NDRG4-B and NDRG4-H demonstrated different responses to RA treatment. The expression of NDRG4-H was increased, whereas the expression of NDRG4-B was not changed by RA treatment. Conclusions NDRG4 may be related to the development and growth of the neurons of central nervous system. Further study of NDRG4 might be beneficial for understanding the pathogenesis of neurodegenerative disorders, such as Alzheimer's disease, etc.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2003年第12期735-737,共3页
Chinese Journal of Geriatrics
基金
国家自然科学基金资助 (3 0 0 70 3 0 5 )
