摘要
目的 观察正常人外周血单个核细胞 (peripheralbloodmononuclearcells ,PBMNCs)及几种肿瘤细胞株经6 0 Co源照射后其细胞周期阻滞现象的动态变化。方法 用6 0 Co源分别以 6,10 ,15Gy的吸收剂量照射正常人PBMNCs及HL 60、K562、SiHA和 113肿瘤细胞株。并于照射后 6,12 ,2 4,48及 60h以流式细胞仪测量其细胞周期变化。结果 正常终末细胞的细胞周期基本处于停滞状态 ,95%细胞均处于G1期 ,当其接受照射后依然表现周期停滞状态 ;除 113细胞表现G1期阻滞外 ,其他肿瘤细胞在受到照射后 ,均表现G2 M期阻滞 ;HL 60放射敏感性较SiHA强。结论 不同种类细胞对放射线表现的细胞周期阻滞现象不同 。
Objective To observe the cell cycle arrest changes in peripheral blood mononuclear cells ( PBMNCs) of normal persons and several kinds of tumor cell lines after 60 Co γ-irradiation. Methods PBMNCs of normal persons,HL-60,K562,SiHA and 113 tumor cell lines were irradiat ed with 60 Co γ-rays at the absorbed doses of 6,10,15 Gy.Cell cycles changes were checked 6,12,24,48 and 60 h after the irradiation. Results A stasis state was observed in normal person PBMNCs, 95 p ercents of which were in G-1 phase,and they still remained stasis after the irr adiation.Except the 113 cell line manifesting G-1 phase arrest,all other tumor cell lines showed G-2/M phase arrest after irradiation.The radiation sensitivit y of HL-60 was higher than that of SiHA cell line. Conclusion Different cell lines have different cell cycle arrest r eaction to radiation and their radiation sensitivity are also different.;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2003年第6期418-420,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金资助项目 ( 3980 0 1 49)
