摘要
目的 探索诱导形成稳定的混合型嵌合体。方法 异种鼠实验模型分3组。受体接受致死剂量全身照射后输入来自同种和/或异种的骨髓,其中 A组(n=14)不做处理;B组(n=14)经中波紫外线(UVB)处理;C组(n=8)对照,检测嵌合体等。结果 当UVB强度≥1.5 mW/cm^2时,骨髓不能植入;混有 5×10~6的同种骨髓时,异种骨髓细胞数≥500×10~6方可植入,否则即被排斥;UVB处理后,无论细胞数如何只有同种骨髓植入。结论 异种和同种骨髓细胞存在竞争植入,且以后者优势;无论处理与否,均未获得混合型嵌合体;异种骨髓细胞可暂时支持同种骨髓的植入;经处理后的异种骨髓可防止移植物抗宿主病(GVHD)的发生。
Objective To find a way of inducing stable mixed chimerism. Methods Recipients
(Lewis rat) were conditioned with a lethal dose of total-body irradiation (1100 cGy) and reconstituted
with a mixed xenogeneic and syngeneic bone marrow (B10Br + Lewis to Lewis). The experimental mod-
els of xenogeneicmice were divided into group A without UVB pretreated, group B with UVB pretreated
and group C as control. Chimerism and others were determined. Results The bone marrow could not be
implanted when UVB 1. 5 mW/cm^2 was given. With 5×10~6 syngeneic bone marrow, the 500×10~6 were
necessary for xenogeneic bone marrow to survive. But with UVB pretreated, only syngeneic ones grew.
Conclusion Both donor and host bone marrow cells competed with each other for survival when injected
together in a lethal irradiated recipient. The host cells had a competitive advantage over the donor cells.
Wheather the xenogeneic bone marrow was pretreated with UVB or not, no mixed chimersim was ob-
tained. It was also noted that the xenogeneic bone marrow supported the recipient alive who would have
died if given less than 100×10~6 host cells. At higher xenogeneic cell dose at which we had experienced
GVHD previously, UVB pretreatment could prevent GVHD.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第12期1133-1134,共2页
Chinese Journal of Experimental Surgery
