摘要
目的 探讨DNA甲基转移酶(DNMT)1、3a、3b mRNA在膀胱癌组织中的表达及其意义。方法 采用逆转录-聚合酶链反应(RT-PCR)技术检测 DNMT1、3a、3b mRNA在40例人膀胱癌组织及31例人正常膀胱粘膜组织中的表达。结果 膀胱癌组织中DNMT1、3b表达的阳性率、表达值均高于正常对照组(P<0.05);DNMT3a的表达与对照组相比,差异无显著性(P>0.05);3种基因的表达率与表达值在不同肿瘤分级、分期、生长方式及原发肿瘤与复发肿瘤之间差异无显著性(P>0.05);DNMT1与DNMT3b表达值明显相关(P<0.01)。结论 DNMT1、3b表达升高是膀胱癌形成、发展中的早期、普通事件,可作为早期诊断和复发监测的有效指标,DNMT1与DNMT3b的表达可能存在共同调节机制。
Objective To evaluate the significance of aberrant DNA methyltransferase (DNMTs)
expression in human bladder carcinoma. Methods The levels of mRNAs encoding DNA methyltransfer-
ases were measured by reverse transcription followed by semi-quantitative detection of PCR products. Re-
sults The positive expression rate and the average level of mRNA for DNMT1 and DNMT3b in bladder
cancers were all significantly higher than in normal bladder mucosae (P < 0. 05), whereas the expression
of DNMT3a in the two groups showed no significant difference (P > 0. 05). There was no significant
correlation between the expression of DNMTs and the clinicopathological parameters of tumors (histologi-
cal classification, clinical stage, morphological type of tumors, primary case or recurrence case, P > 0. 05).
Over-expression of DNMT1 mRNA was significantly associated with that of DNMT3b (P < 0. 01). Con-
clusion Both over-expression of the maintenance DNA methyltransferase DNMT1 and over-expression
of de novo DNA methyltransferase, DNMT3b, are involved in human bladder carcinogenesis. There could
be a certain co-regulation mechanism between DNMT1 and DNMT3b.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第12期1071-1072,共2页
Chinese Journal of Experimental Surgery
