一种改进的微量体液免疫功能测定法

A Modified Microquantitative Method of Humoroimmunity Assay

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摘要本文作者对传统的体液免疫功能测定方法进行了多方面的改进,使有绵羊红细胞、致敏动物血清及补体参加的体外溶血反应在96孔培养板中进行。作者通过选择合适的比色波长及应用MINIREADER Ⅱ,SERIES小型电子分光光度计,使这种新改进的实验方法的检测灵敏度有了很大的提高,这种改进后的新方法使实验操作步骤变得更加简单易行,同时各种试剂用量也大大地减少。该方法的测定结果准确、客观,并有良好的重复性,它能给药物筛选工作及其它方面的体液免疫研究带来诸多方便。 The methods of conventional humoral immunity assay were modified by the au-thor in several aspects. The new method of hemolysis test in vitro utilizes 96 holeplate instead of test tubes. In the new method determinable sensitivity is raised enor-mously, operation becomes simpler and easier by means of choosing suitable colorimetricwavelength and applying MINI-READER Ⅱ, SERIES 2(small-sized electric spectro-photometer), made in DYNATECH LABORATORIES, INC USA. Meanwhile, theconsumptive reagents using in the new method are much less than conventional methods.The results of the new methed are more accurrate and more reproducable. Cy-colphosphamidum as a control drug has been used in resting experiment with newdrug screening work, it has been verified that the new method is trustworthy. Theresults show that the new method is convenient and accurate for immunologicalresearch.