摘要
目的 :探讨p16基因甲基化在急性淋巴细胞白血病发生机制中的作用。方法 :采用限制性内切酶酶切结合多聚酶链反应 (PCR)方法检测 82例急性淋巴细胞白血病患者白血病细胞p16基因部分启动子和外显子 1CpG岛甲基化的情况 ,同时应用免疫组织化学染色法检测p16蛋白的表达。结果 :31例急性淋巴细胞白血病患者存在p16基因的甲基化 ;1例p16基因纯合缺失 ;39名患者p16蛋白表达阴性 ;p16蛋白阴性表达患者的初次化疗完全缓解率和平均生存时间低于p16蛋白阳性表达者。结论 :p16基因甲基化在急性淋巴细胞白血病的发生机制中有一定的作用 ,p16蛋白可能是评估急性淋巴细胞白血病预后的一个指标。
Objective: To investigate the effect of methylation and expression of gene p16 on pathogenesis of acute lymphoblastic leukemia. Methods: PCR-based methylation assay and immunohistochemistry technique were applied to test whether there were methylation or expression of p16 gene in 82 patients with acute lymphoblastic leukemia. Results: Among the 82 patients tested, one homozygous deletion was found in a case and 31 cases (38.3%) were found to have methylation of p16 gene. Besides, there were still 39 cases without p16 protein expression. Patients with expression of p16 protein had a higher complete remission (CR) rate after the first chemotherapy and a longer survival time than those of patients without expression of p16 protein. Conclusion: Methylation of p16 gene probably plays a role in the pathogenesis of acute lymphoblastic leukemia and p16 protein may have some value as a prognostic index.
出处
《贵阳医学院学报》
CAS
2003年第6期496-500,共5页
Journal of Guiyang Medical College
