摘要
目的 研究早期大肠癌中COX 2的表达及其临床意义。方法 使用免疫组化染色法分别检测了 32例早期大肠癌外科术后石蜡标本、33例大肠腺瘤、18例正常大肠粘膜活检组织中COX 1和COX 2蛋白的表达。结果 依表达程度由 ( )至 (+++)四级计算 ,COX 2的表达率在正常结肠粘膜中分别为 83.3%、16 .7%、0 %、0 % ;在结肠腺瘤中分别为 12 .1%、4 2 .4 %、36 .4 %、9.1% ;早期大肠癌中分别为 6 .3%、2 8.1%、4 6 .9%、18.7%。早期大肠癌、大肠腺瘤中COX 2的表达率均明显高于正常粘膜 (P <0 .0 1) ,但早期大肠癌与大肠腺瘤中COX 2的表达率无显著性差异。COX 2的表达与早期大肠癌、大肠腺瘤各项被研究的临床病理特征无关。COX 1在早期大肠癌 ,大肠腺瘤及正常肠粘膜中呈低水平表达。结论 COX 2的表达在由正常大肠粘膜至大肠腺瘤、早期癌的发展过程中呈上调趋势。COX 2的表达是大肠肿瘤形成过程中的早期事件 ,不能作为大肠癌早期诊断的癌标记物。
Objective To study the expression of COX-2 in the early colorectal cancers(ECCs) and its clinic significance. Methods COX-2 expression was examined by immunohistochemical staining in thirty-two early colorectal cancer specimens surgically resected, thirty-three colorectal adenoma and eighteen normal colorectal mucosal tissues taken by endoscopy biopsy.Results COX-2 expression, divided into 4 grades from ”-”to“”, is respectively 83.3%、16.7%、0%、0% in normal colonic mucosal tissues; 12.1%、42.4%、36.4%、9.1% in colorectal adenoma; 6.3%、28.1%、46.9%、18.7% in ECCs. The differences in the COX-2 expression between ECCs and normal colorectal mucosae as well as between colorectal adenoma and normal colorectal mucosae were statistically significant (P<0.01).But there was no significant difference between ECCs and colorectal adenoma. There is no relationship between expression of COX-2 and clinicopathological features of either ECCs or colorectal adenoma.COX-1 is only constitutively expressed at low levels in the ECCs, colorectal adenoma and normal colorectal mucosae. Conclusion COX-2 expression is gradually up-regulated in the development course of normal epithelium to colorectal adenoma and early colorectal cancer.COX-2 expression is a early incidents in the pathogenesis of colorectal cancer, and it can not be considered as a marker to diagnose ECC.
出处
《肿瘤》
CAS
CSCD
北大核心
2003年第6期497-499,共3页
Tumor
