摘要
目的:探讨老龄大鼠局灶性脑缺血后CyclinD1表达与小胶质细胞活化、神经元凋亡之间的内在关系。方法:建立光化学诱导的老龄大鼠局灶性脑缺血模型,利用原位分子杂交、IsolectinB4免疫组织化学和原位末端标记等方法,检测缺血4、24 h和3、5 d组大鼠脑组织中的CyclinD1 mRNA阳性细胞、小胶质细胞和凋亡细胞的时空分布规律。结果:CyclinD1 mRNA阳性细胞、小胶质细胞和凋亡细胞皆分布于缺血灶周围,发布空间相互重叠。CyclinD1 mRNA在多种形态细胞中表达,以胶质细胞最为多见,其表达高峰与小胶质细胞活化高峰一致。在各时间点的病灶周围皆发现TUNEL阳性细胞,其高峰时点提前于CyclinD1 mRNA阳性细胞及小胶质细胞活化高峰。结论:CyclinD1可能参与了缺血后小胶质细胞的活化及神经元的凋亡过程。
Aim:To investigate the relationship between CyclinDl expression and microglia activation and neuron apoptosis after focal cerebral ischemia in aged rats.Methods:The focal cerbral ischemia models induced by photochemistry in aged rats were established. Using situ hybridization, histochemical staining with isolectin-B4 and TUNEL staining, the density and distribution of CyclinDl mRNA positive cells, microglia and apoptotie cells 4 h,24 h,3 d,5 d after focal eerbral ischemia were observed. Results: CyclinDl mRNA positive cells, microglia and apoptotie cells were present in perifocal area in different time points after ischemia. CyclinDl mRNA expressed in many forms of nerve cells but mainly expressed in neuroglia cells and the expression peak was consistant with that of microglia. The TUNEL-positive cells were present from 4 h to 5 d after ischemia and its expression peak was ahead of CyclinDl mRNA positive cells and microglia. Conclusion: CyclinDl may participate in the process of microgha activation and neuron apoptosis after focal eerbral ischemia.
出处
《中国临床神经科学》
2003年第4期343-345,共3页
Chinese Journal of Clinical Neurosciences
基金
国家自然科学基金(39770810)
