摘要
目的 探讨宫内缺血缺氧新生鼠下丘脑及外周血孤啡肽含量的变化及意义。方法 采用放射免疫测定法 ,测定 19只正常新生鼠 (对照组 )、2 1只宫内缺血缺氧 10min新生鼠 (A组 )及 17只宫内缺血缺氧 2 0min新生鼠 (B组 )下丘脑及外周血孤啡肽的含量。结果 (1)B组新生鼠下丘脑及外周血孤啡肽的含量分别为 (114± 2 1)pg/g及 (5 8± 11)ng/L ,A组新生鼠下丘脑及外周血孤啡肽的含量分别为 (71± 14 )pg/g及 (31± 7)ng/L ,对照组新生鼠下丘脑及外周血孤啡肽含量分别为 (48± 9)pg/g及 (19± 4 )ng/L ;A、B两组新生鼠下丘脑及外周血孤啡肽含量均显著高于对照组 (P <0 0 1,P <0 0 5 ) ;且B组新生鼠下丘脑及外周血孤啡肽含量又显著高于A组 (P <0 0 5 )。 (2 )A、B两组新生鼠Apgar评分均显著低于对照组 (P <0 0 1) ,其中B组新生鼠Apgar评分显著低于A组 (P <0 0 5 )。结论 孤啡肽与围产期缺血缺氧的发生、发展密切相关。
Objective To evaluate the role of orphanin in the perinatal ischemia-hypoxia. Methods The concentration of hypothalamus and peripheral orphanin were measured by radioimmunoassay . Animal model of perinatal ischemia-hypoxia was set up by ligating uterine vessels. All the rats were delivered by cesarean section and scored according to respiration,heart beat,skin color,muscle tone and reflex after delivery. Results (1)The levels of orphanin in hypothalamus and peripheral blood in group B were (114±21)pg/g and(58±11)ng/L respectively. In group A,the were (71±14) pg/g and(31±7)ng/L respectively , the levels of orphanin in group B increased significantly when compared with the group A( P <0.05). In control group,the levels of orphanin were(48±9)pg/g and(19±4)ng/L .The levels of orphanin in group A and B were significantly higher than those in the control group( P <0.01, P <0.05). (2) The Apgar scores in groups A and B were significantly decreased than that in control group ( P < 0.01). The group A pups had significantly better scores than the group B ( P <0.05). Conclusion The perinatal ischemia and hypoxia was associated with orphanin,the changes of orphanin levels may play an important role in the pathophysiological changes in perinatal ischemia and hypoxia.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2003年第11期683-684,共2页
Chinese Journal of Obstetrics and Gynecology
