摘要
目的 :探索不同人群中抗SARS冠状病毒特异性抗体的发生、发展及分布规律 ;评价间接ELISA方法检测SARS冠状病毒IgG、IgM特异性抗体试剂盒及改进方法。 方法 :分别获取SARS流行前无偿献血者标本 3990份 ,一线抗SARS医务人员标本 397份和临床确诊的SARS患者标本 15 7份 ,采用ELISA方法进行SARS冠状病毒特异性抗体检测。结果 :SARS流行前无偿献血者标本 3990份共检出IgG抗体阳性标本 16份 ,阳性率为 0 .4 0 % ;IgM抗体阳性标本 0份 ,阳性率为 0 %。一线抗SARS医务人员标本 397份 ,共检出IgG抗体阳性标本 2份 ,阳性率为 0 .5 0 % ;IgM抗体均为阴性。临床确诊的SARS患者标本 15 7份 ,共检出IgG抗体阳性标本 119份 ,阳性率为 75 .79% ;IgM阳性标本 6 8份 ,阳性率为 4 3.31%。结论 :一线抗SARS医务人员与SARS流行前无偿献血者IgG、IgM抗体阳性率无显著差异 (P =0 .76 >0 .0 5 ) ;采用该SARS冠状病毒 (变异株 )IgG抗体试剂盒检测SARS流行前无偿献血者人群存在一定的阳性率 ,说明该试剂盒包被的抗原与其它免疫球蛋白 (IgG)有交叉反应 。
Objectives:To explore the generation, development and distribution of anti-SARS (severe acute respiratory syndrome) specific antibodies in different populations; To search the improvement on the reagent kits. Methods:Serum collected from 3 990 volunteer blood donors before the outbreak of SARS were assayed. Meanwhile, 397 health care workers from SARS wards and 157 cases of SARS patients were parallelly assayed by ELISA methods. Results:Anti-SARS specific IgG antibody was found in 16 donors among 3 990 volunteer blood donors. The positive rate was 0.40%. No specimen was positive with IgM antibody. Of 397 health care workers, 2 person had IgG antibody, and the positive rate was 0.5%. No IgM antibody was determinated in 397 health care workers. In 157 SARS cases, IgG antibody was found in 119 patients. The positive rate became 75.79%. However, IgM antibody was found only in 68 SARS patients. The positive rate was 43.31%. Conclusion:The difference of IgG and IgM antibody positive rate were not significant between volunteer blood donors and health care workers from SARS wards(P=0.76>0.05). Certain positive rate exist when this kind of reagent kits were used to determinate IgG antibody, which showed that the antigen coated on the micro-plate cross reacted with other kind of IgG antibody. It seems that the reagent kits need some improvement.
出处
《军医进修学院学报》
CAS
2003年第4期262-264,共3页
Academic Journal of Pla Postgraduate Medical School
基金
解放军总医院院长基金资助项目 ( 2 0 0 3SARS 13)
