摘要
目的 探讨 α1 -肾上腺素受体拮抗剂乌拉地尔 (urapidil)对低氧性肺动脉高压的预防作用。方法 将2 1只雄性 SD大鼠分为正常对照组、低氧组和低氧 +乌拉地尔组 ,以常压低氧 3周建立大鼠肺动脉高压模型 ,分别称量大鼠心脏右心室 (RV)和左心室加室间隔 (L V+S)质量 ,计算出右心室肥厚指数 RV/ (L V+S) ,并对大鼠肺组织切片进行 HE染色 ,采用图象分析技术测定大鼠肺小动脉管壁厚度 (WT)和面积 (WA) ,计算出肺小动脉管壁厚度指标——管壁厚度占外径的百分比 (WT% )和管壁面积占总面积的百分比 (WA% )。结果 低氧组大鼠 RV/(L V+S)、WT%和 WA%分别为 (33.0 6± 1.74 ) % ,(2 5 .15± 1.4 7) %和 (73.88± 2 .93) % ,对照组为 (2 3.2 1±2 .31) % ,(13.5 5± 1.6 0 ) %和 (4 8.2 3± 4 .4 3) % ,两组之间差异有显著意义 (P均 <0 .0 1) ;而经乌拉地尔处理的低氧大鼠则分别为 (2 6 .6 3± 1.4 4 ) % ,(14 .5 7± 2 .2 7) %和 (5 2 .6 8± 3.98) % ,均低于低氧组 (P<0 .0 1)。结论 α1 -肾上腺素受体参与了低氧性肺动脉高压的形成过程 ,其拮抗剂乌拉地尔对低氧性肺动脉高压具有明显的预防作用。
Objective To investigate the preventive effect of urapidil, an α 1-adrenoceptor antagonist, on hypoxic pulmonary vascular remodeling. Methods Twenty-one male Sprague-Drawley rats were randomly allocated into three groups: control group, hypoxic group and hypoxic plus urapidil group. The animal model of hypoxic pulmonary hypertension was established by exposing the rats to normobaric hypoxic condition for three weeks, and the rats of hypoxic plus urapidil group were treated with urapidil 10 mg/kg before and after hypoxic processing. The right ventricle (RV) and left ventricle plus septum (LV+S) of rat were weighed respectively, and the RV/(LV+S) was calculated. The wall thickness(WT) and wall area(WA) of pulmonary arterioles wall were measured by a computerized image analyzer, and the index of wall thickness of rats pulmonary arterioles-the percentages of the wall thickness in the external diameter (WT%) and the wall area in the total vascular area (WA%) were calculated. Results In the hypoxic group, RV/(LV+S) ,WT% and WA% were (33.06± 1.74)% , (25.15±1.47)% and (73.88±2.93)% respectively, while in the control group, they were (23.21± 2.31)% , (13.55±1.60)% and (48.23±4.43)%, the difference between the two groups was significant (P<0.01). In the hypoxic plus urapidil group, they were (26.63±1.44)%, (14.57±2.27)% and (52.68±3.98)%, being remarkably decreased as compared with those in the hypoxic group (P<0.01). Conclusion Urapidil can prevent hypoxic pulmonary hypertension.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第1期71-73,共3页
Journal of Sichuan University(Medical Sciences)
