摘要
利用构建的高选择型基因工程菌生物富集模拟电解废水中的汞离子.模拟电解废水中除含有3.0mg·L-1的汞离子外,还含有十种以上的其它金属离子,且pH值为9.0.实验表明,与重组菌对只含汞离子的水溶液的处理结果比较,电解废水中其它组份的存在意外地增大了重组菌富集汞离子的作用速率,但同时却使细菌的最大汞富集量降低了约30%.汞水溶液pH的变化对重组菌的汞富集行为影响很小,说明该基因工程菌能在很宽的pH范围内有效地富集汞.实验还考察了EDTA及离子强度对富集行为的影响.
Bioaccumulation performance of mercury from simulated electrolyte wastewater by genetically engineered E.coli BL21(DE3), which expresses a mercurial ion transport system and metallothionein is investigated. These are more than 10 components existing in the simulated wastewater besides 3.0 mg·L^(-1) mercury, and the pH of the wastewater is 9.0. The co-ions didn't decrease but significantly promoted mercury uptake rate of induced cells, while a 30% loss of the amount of mercury bioaccumulation is obtained in comparison with that from solution only containing mercury. The varying of pH in wastewater only slightly drops mercury accumulation, suggesting, induced cells has a property of pH insensitivity.
出处
《泉州师范学院学报》
2003年第6期71-75,共5页
Journal of Quanzhou Normal University
基金
泉州市科技计划重点项目(Z200234)
