摘要
用转座子标签技术克隆了位于农杆菌 (A 2 0 8株 )染色体上的致病基因acvB、abvA、chvA ,简单介绍克隆技术的研究思路和策略。染色体基因是农杆菌吸附到植物细胞表面所必需的基因 ,当某一基因发生突变时 ,就不能完成贴壁反应而失去毒性。由于转座子Tn5的插入 ,导致染色体毒性基因失活 ,最终使农杆菌感染后的受体细胞不能致瘤。用实验证据阐述各基因在T DNA形成、转移、整合、表达等过程中担负的重要作用。对延宕至今的T DNA穿壁问题作了推测 :植物细胞壁表面可能存有T DNA的天然穿壁孔道。
Chromosomal virulence genes acvB, abvA, chvA of %Agrobacterium tumefaciens% were cloned with the technique of transposon 5 insertion. The chromosome genes are necessary for %Agrobacterium tumfaciens% absorbing to cell ular surface of plant, the adherence reaction can't be executed and result in losing the toxicity if mutations are occurred in some chromosome genes. The chromosome toxicity gene is inactivated due to transposon Tn5 be inserted and the accept ant cell infected with Agrobacterium tumefaciens can't cause tumor ultimately.This article briefly introduces the research way of thinking and strategy of this technique and the important roles of every gene, which are taken of in the process of T-DNA's form, transfer, integration, and expression %etc%. This article also gives a presumption to T-DNA's transport: The plant cell wall's porin may be T-DNA's natural channel.
出处
《生物工程学报》
CAS
CSCD
北大核心
2003年第6期651-654,共4页
Chinese Journal of Biotechnology
基金
国家教育委员会国家留学基金资助 (No .9983 2 0 77)
