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家蚕核型多角体病毒山东株p35基因的克隆及序列分析

Cloning and Sequencing of p35 Gene from Bombyx mori Nuclear Polyhedrosis Virus Strain Shandong
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摘要 根据家蚕核型多角体病毒T3株p35基因非编码区序列设计一对特异性引物,应用PCR技术从家蚕核型多角体病毒山东株中扩增得到一条1080bp的片段。测序结果表明,该片段含有p35基因,开放阅读框长900bp,编码299个氨基酸,分子量为34.91kDa,pI=6.4。序列分析表明,BmNPV山东株的p35基因与T3株存在差异。 According to the 5′ and 3′ untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3,a pair of primers was designed employing computer analysis.With the primers,polymerase chain reaction (PCR) was performed and a 1 080 bp fragment was obtained from BmNPV strain Shandong.Sequencing result showed that it was p35 gene (GenBank accession number:AY157746),and it included an open reading frame of 900 bp,encoding 299 amino acids with Mr=34.91kDa and pI=6.40.BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.
作者 姜丽华 钟万芳 蔡平钟 徐兴耀 阎文昭
机构地区 华南农业大学蚕丝科学系 四川省农业科学院生物技术育种工程中心
出处 《遗传》 CAS CSCD 北大核心 2003年第6期681-684,共4页 Hereditas（Beijing)
基金 十五"国家科技攻关专题(2001BA502B01-02-03) 四川省生物技术重点资助项目
关键词 家蚕核型多角体病毒 山东株 P35基因 克隆 序列分析 Bombyx mori nuclear polyhedrosis virus strain Shandong p35 gene clone sequence analysis
分类号 S852.65 [农业科学—基础兽医学]
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2003年 第6期

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