摘要
(1)对长春地区兔、小鼠、牛以及婴幼儿腹泻的隐孢子虫感染情况作了调查,其感染率分别为36%、90%、9/10和1/22;(2)对兔隐孢子虫卵囊排出规律的观察结果表明,每隔6～8d出现一个高峰期,在两个高峰期之间有时检不到卵囊;(3)交叉感染试验,从兔粪便中分离的卵囊经口感染BALB/c乳鼠和雏鸡后,在鼠粪便中检到卵囊,而在鸡粪便中未发现卵囊;(4)建立了兔和鼠的隐孢子虫病动物模型;(5)成功地进行了隐孢子虫体外培养试验;(6)鼠隐孢子虫病的治疗药物筛选结果,10^(-3)mol/L SM520和10^(-6)mol/LSMW在体外对隐孢子虫有杀灭作用;动物试验发现,中药配方I和SMW对鼠隐孢子虫有一定抑制作用.
(1) The infective rates of Cryptosporodium parvum in rabbits, mice, cattle and infants investigated firstly in Changchun region, Jilin province were 36%, 90%, 9/10 and 1/ 22, respectively. (2) Oocyst expulsion from infected animals occurs regularly every 6-7 days. (3) Oocysts of Cryptosporodium parvum isolated from rabbits can infect sucking mice but can not infect chicks by oral route. (4) Rabbits and mice which have been given with Dexamethasone may be used as animal model of cryptosporidiosis. (5) C. maris and C. parvum isolated from rabbits and mice were successfully cultivated in HEL cells and chicken embryos. (6) The screening test of therapeutic drugs for cryptosporidiosis indicated that 10-3 mol/L SM520 or 10-6 mol/L SMW could kill the cryptosporodia in vitro and that the prescription I of Chinese traditional medicine plus SMW has a certain in vivo static effect on Cryptosporodium muris.
关键词
隐孢子虫
体外培养
生物学特性
Cryptosporodium
cryptosporidiosis
animal model
cultivation
