摘要
对30例40日龄自然感染弯曲菌性肝炎鸡的肝脏、胆囊及主要脏器进行了眼观、病理组织学、肝脏的透射电镜、胆囊的扫描电镜和透射电镜的观察.根据病变特点分为急性肝炎和亚急性肝炎.30例患鸡肝脏石蜡切片应用免疫过氧化物酶染色,空弯菌的检出率为100%;肝脏断面触片应用石炭酸复红稀释液及Warthin-Starry染色时,可检出弧形、弯曲状或螺旋状病菌.提出了以肝脏肉眼变化,结合肝断面触片石炭酸复红稀释液染色检出弯曲菌,作为宰后快速检验方法.
The livers, gallbladder and main organs of 30 chicken at age of 40 days infected naturally with campylobacter hepatitis were observed macropathologically and histopathologi-cally, and some livers and gallbladders were further observed ultrastructurally. According to the pathological findings, campylobacter hepatitis of chicken may be classified into acute and sub-acute forms. In the liver sections of all these cases stained with Warthin-Starry method, the campylobacter like organisms were found and they were identified as Campylobacter jejuni with immunoenzymatic histochemical technique. Besides, the same organisms were also found in the impression smears of incised livers of 90% cases stained with dilute carbol fuchsin. The routine procedures for chicken carcass examination were proposed.
关键词
鸡
弯曲菌性
肝炎
病理学
chicken
campylobacter hepatitis
Campylobacter jejuni
pathology
