摘要
目的：探讨肝细胞生长因子／扩散因子（ Ｈ Ｇ Ｆ／ Ｓ Ｆ）、转化生长因子β（ Ｔ Ｇ Ｆβ）在结核性胸腔积液（ Ｔ Ｂ Ｐ Ｅ）中的表达，寻找诊断结核性胸膜炎的特异性标记。方法：用 Ｈ Ｇ Ｆ／ Ｓ Ｆ Ｅ Ｌ Ｉ Ｓ Ａ 药盒和生物法测定３２ 例胸腔积液中 Ｈ Ｇ Ｆ／ Ｓ Ｆ和 Ｔ Ｇ Ｆβ水平，并对 Ｔ Ｂ Ｐ Ｅ组中１０ 例病人同时作血清 Ｈ Ｇ Ｆ／ Ｓ Ｆ、 Ｔ Ｇ Ｆβ检测。结果： Ｈ Ｇ Ｆ／ Ｓ Ｆ在 Ｔ Ｂ Ｐ Ｅ中为７．５±７．３ ｎｇ／ｍ ｌ，高于非 Ｔ Ｂ Ｐ Ｅ的０．９８±０．８８ ｎｇ／ｍ ｌ（ Ｐ＜ ０．０５）。１０ 例 Ｔ Ｂ Ｐ Ｅ者的胸水和血清 Ｈ Ｇ Ｆ／ Ｓ Ｆ含量分别是１０．９±６．６ ｎｇ／ｍ ｌ和１．９±１．７ｎｇ／ｍ ｌ（ Ｐ＜ ０．００２）。 Ｔ Ｇ Ｆβ在 Ｔ Ｂ Ｐ Ｅ中含量为２７．３±１１．１ ｎｇ／ｍ ｌ，高于其在血清中的含量３．９±３．３ ｎｇ／ｍ ｌ（ Ｐ＜ ０．００１），也高于对照血清的１．８７±１．０５ ｎｇ／ｍ ｌ（ Ｐ＞ ０．０５）。结论：在 Ｔ Ｂ Ｐ Ｅ中存在高水平 Ｈ Ｇ Ｆ／ Ｓ Ｆ、 Ｔ Ｇ Ｆβ。这可能与结核性肉芽肿形成有关。
Objective:To investigate the expression of hepatocyte growth factor/scatter factor (HGF/SF) and transforming growth factor β (TGF β) in tuberculous pleural effusions (TBPE) and to search for specific diagnostic marker.Methods:Specific HGF/SF kit and growth inhibition bioassay were used to detect the expression level of HGF/SF and TGF β in 32 specimens of pleural effusion.The serum level of HGF/SF and TGF β in 10 TBPE patients were also examined.Results:The expression level of HGF/SF in TBPE was 7.5±7.3 ng/ml,higher than that in nontuberculous pleural effusion 0.98±0.88 ng/ml ( P <0.05).HGF/SF levels in 10 TBPE and their self controlled serum were 10.9±6.6 ng/ml and 1.9±1.7 ng/ml respectively ( P <0.002).TGF β level in TBPE was 27.3 ±11.1 ng/ml,higher than serum levels of TBPE 3.9±3.3 ng/ml ( P <0.001) and controls 1.87 ±1.05 ng/ml ( P >0.05).Conclusion:High levels of HGF/SF and TGF β in TBPE may exert some pathophysiological activities in the development of tuberculous granulomatous inflamation,and play important roles in the promotion of pleural fibrosis.
出处
《浙江大学学报（医学版）》
CAS
CSCD
1999年第4期154-157,共4页
Journal of Zhejiang University(Medical Sciences)
