摘要
目的:比较两种常用的人乳头瘤病毒(HPV)的检测方法。方法:第二代杂交捕获法(HCⅡ)和聚合酶链反应(PCR)检测妇女宫颈分泌物中的高危型HPV。结果:103例宫颈病变患者中,PCR和HCⅡ检测HPV的阳性率分别为41.7%和39.9%。两种检验方法具有相关性(P<0.05)。结论:PCR和HCⅡ具有较好的一致性。
Objective Two commonly used tests for human papillomavirus detection were compared. Methods Hybrid capture Ⅱ (HC Ⅱ ) test and polymerase chain reaction (PCR) were used for detection of high-risk HPV from cervical scrapes of 103 women. Results The overall HPV DNA positivity for known type was 41. 7 % by PCR compared to 39. 9 % by HC Ⅱ. There is significant relationship between HC Ⅱ and PCR (P<0. 05). Conclusion The results show good agreement between HC Ⅱ and PCR assay for HPV.
出处
《实用医技杂志》
2003年第11期1215-1216,共2页
Journal of Practical Medical Techniques