摘要
目的 探讨亚甲基四氢叶酸还原酶 (MTHFR)基因多态性与中国人 2型糖尿病微血管并发症的关系。方法 运用PCR RFLP检测 2 63例中国人 ( 2 0 6例为 2型糖尿病 ,其中 14 8例合并肾病或视网膜病变 ,5 7例为正常对照组 )MTHFR基因C677T位碱其突变 ,比较各组间等位基因频率和基因型频率。结果 ( 1)同时合并肾病和视网膜病变的 2型糖尿病组与无微血管并发症的2型糖尿病组及正常对照组相比 ,TT基因型频率显著增加 ,突变等位基因T频率也明显升高。 ( 2 )2型糖尿病合并肾病组TT基因型频率及T等位基因频率明显高于不伴有肾病的 2型糖尿病组及正常对照组。 ( 3 ) 2型糖尿病合并视网膜病组与无视网膜病的 2型糖尿病及正常对照组相比 ,TT基因型频率及T等位基因频率明显升高。结论 MTHFR基因C677T碱基突变是促进中国人 2型糖尿病患者并发微血管并发症的危险因子 。
Objective To investigate the association between genetic polymorphism of methylenetetrahydrofolate reductase(MTHFR)C677T and diabetic microangiopathy in Chinese population.Methods A case-control study for 263 Chinese subjects(including 206 type 2 diabetes mellitums with or without nephropathy or retinopathy and 57 normal control subjects) was performed.The genetic polymorphism of MTHFR C677T was determined by PCR-RFLP.Results (1)The frequencies of TT genotype and T allele in type 2 diabetes mellitus with both nephropathy and retinopathy were significantly higher than those in type 2 diabetes mellitus without microangiopathyand normal control group.(2)Elevated frequencies of TT genotype and T allele were observed in type 2 diabetes mellitus with nephropathy as compared with type 2 diabetes mellitus without nephropathy and normal control group.(3)In type 2 diabetes mellitus with retinopathy,the increased frequencies of TT genotype and T allele were observed in comparison with type 2 diabetes mellitus without retinopathy and normal control group.Conclusion The genetic polymorphism of MTHFR C677T are associated with the development of diabetic microangiopathy.The T allele may be a risk factor for developing microangiopathy in type 2 diabetes mellitus.
出处
《临床内科杂志》
CAS
北大核心
2003年第12期628-630,共3页
Journal of Clinical Internal Medicine
关键词
糖尿病
亚甲基四氢叶酸还原酶
基因
肾疾病
视网膜病变
TDiabetes mellitus
Methylenetetrahydrofolate reductase
Gene
Nephropathy
Retinopathy
