摘要
大肠杆菌表达系统在红霉素基因工程中的应用面临着许多难题 :聚酮合成酶中ACP的后修饰、6_dEB生物合成前体供应、大环内酯糖基化修饰等。通过向大肠杆菌中转入枯草杆菌磷酸泛酰巯基转移酶基因sfp ,使大肠杆菌中合成的ACP得以后修饰 ;6_dEB生物合成前体丙酰CoA和丙二酰CoA可通过Pfeifer途径和Dayem途径提供 ;目前 ,在大肠杆菌体内已可以合成 6_dEB。利用大肠杆菌系统合成新的大环内酯化合物、对大环内酯糖基化修饰和提高大肠杆菌系统合成大环内酯类抗生素产量将是今后研究的重点。
When E.coli expression system is applied in erythromycin genetic engineering, there are many difficulties to face, such as posttranslation modification of ACP in PKS, supply of precursors for 6-dEB biosynthesis, glycosyl modification of macrolides, and so on. ACP can be modified by means of transforming phosphopantetheinyl transferase gene sfp from Bacillus subtilis into E.coli, and the precursors for 6-dEB biosynthesis, propionyl-CoA and methylmalonyl-CoA,can be provided by Pfeifer pathway and Dayem pathway,and 6-dEB could have been synthesized in E.coli till now. Synthesis of novel macrolide compounds, glycosyl modification of macrolides, and promotion of macrolide antibiotics output with E.coli expression system would be important research fields in the future.
出处
《军事医学科学院院刊》
CSCD
北大核心
2003年第5期381-384,共4页
Bulletin of the Academy of Military Medical Sciences
基金
华北制药集团-军事医学科学院博士后基金
安徽省高等学校优秀中青年骨干教师基金
