摘要
目的 :研究eIF_5A的亚细胞定位 ,为了解其生物学功能提供线索。方法 :借助免疫荧光技术和GFP标签技术 ,用激光共聚焦显微镜检测eIF_5A的亚细胞定位。结果 :免疫荧光检测结果表明 ,eIF_5A定位于细胞质中 ;GFP_eIF_5A瞬时转染后 ,起初eIF_5A为全细胞分布 ,以后逐渐转移到细胞质中。第 5 0位赖氨酸突变为丙氨酸后的eIF_5A则分布于整个细胞。结论 :eIF_5A具有核质穿梭功能 ,它的亚细胞定位是个动态变化过程 ,hypusine可影响其亚细胞定位 ,进而可能影响其功能的发挥。
Objective:To study the subcellular localization of eIF-5A, and find the clues for its function.Methods:eIF-5A subcellular localization was examined by laser confocal microscope, using the approaches of immunofluorescent staining and GFP-tagging.Results:The localization of eIF-5A was primarily cytoplasmic by indirect immunofluorescent staining. The results of transient transfection showed that GFP-eIF-5A was distributed over whole cell at the beginning of its expression, but was gradually concentrated in cytoplasm. The mutation of lysine 50→alanine affected its distribution in the cytoplasm.Conclusion:eIF-5A might be a nucleocytoplasmic shuttle protein, and its subcellular localization was a dynamic process and hypusine could affect the function of eIF-5A through swaying its subcellular localization.
出处
《军事医学科学院院刊》
CSCD
北大核心
2003年第5期330-333,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家"8 6 3"生物高技术基金资助项目 (2 0 0 1AA2 33041)
国家自然科学基金资助项目 (30 0 70 377
30 2 70 2 99)
关键词
EIF-5A
亚细胞定位
免疫荧光
激光共聚焦
eIF-5A
hypusine
subcellular localization
immunoflourescence
laser confocus
