摘要
目的:研究ChAT,GAD65和PKC酶活性在慢性胰腺炎和胰腺癌组织中表达特征及其临床病理意义。方法:胰腺癌(n=47)和慢性胰腺炎(n=10)手术切除标本经40g/L中性甲醛固定后常规制作石蜡包埋切片,ChAT,GAD65和PKC酶活性表达染色方法均为常规ABC免疫组化法。结果:胰腺癌ChAT,GAD65和PKC表达阳性率(48.9％,55.3％和57.4％)及其评分(2.2±1.4,2.2±1.2和2.1±1.6)明显高于慢性胰腺炎阳性率(0％,10.0％和10.0％)及其评分(0.2±0.4,0.6±0.9和0.6±0.9),均有显著或高度显著性差异(P<.05或P<0.01).高分化腺癌ChAT评分值明显高于低分化腺癌(P<0.05),但阳性率之间无明显差异(P>0.05);高分化腺癌GAD65,PKC表达阳性率及其评分明显低于低分化腺癌,均有显著或高度显著性差异(P<0.05或P<0.01)。酶活性表达与胰腺癌患者性别、年龄、有无转移等临床特征均无明显关系。GAD65评分与PKC评分存在高度密切关系(r=0.50,P<0.01)结论。ChAT,CAD65和PKC酶活性表达特征可能与胰腺癌发生发展及生物学行为有密切关系,均为胰腺癌重要生物学标志物。
AIM: To study on the expression characteristics of ChAT GAD65 and PKC enzymatic activities and their clinicopatho- logical significance in the tissues of chronic pancreatitis and pancreatic carcinoma. METHODS: The enzymatic activities of ChAT, GAD65 and PKC were detected by immunohistochemical method of avidin-biotin complex on formalin-fixed and routine paraffin- embedded sections of specimens of chronic pancreatitis (n=10) and pancreatic carcinoma (n=47). RESULTS: The positive rate and the score of ChAT, GAD65 and PKC were significantly lower in 10 cases of chronic pancreatitis than that of pancreatic carcinoma (ChAT, 0% vs 48.9%, 0.2±0.4 vs 2.2±1.4; GAD65, 10.0% vs 55.3%, 0.6±0.9 vs 2.2±1.2; PKC, 10.0% vs 57.4%, 0.6±0.9 vs 2.1±1.6). The score of ChAT was significantly higher in well-differentiated adenocarcinoma than that of poorly- differentiated adenocarcinoma (P<0.05). The positive rate and the score of GAD65 or PKC were significantly lower (GAD65, P<0.05; PKC, P<0.01) in Cases of well-differenti- ated adenocarcinoma than in cases of poorly-differentiated. No difference was found for the enzyme expressions and the clinicopathological characteristics among different sex, age, with or without metastasis of pancreatic carcinoma. A highly positive correlation was found between the scores of GAD65 and PKC in pancreatic carcinoma. CONCLUSION: The expression of enzymatic activities of ChAT, GAD65 or PKC might be related to the carcinogenesis, progression and biological behaviors of pancreatic carcinoma. They might be important biological markers of pancreatic carcinoma.
出处
《世界华人消化杂志》
CAS
2003年第10期1554-1557,共4页
World Chinese Journal of Digestology
