摘要
目的 了解福建省宁化林区斑点热自然疫源地存在情况。方法 用聚合酶链反应对该林区鼠、蜱类中感染斑点热群立克次体 (SFGR)进行扩增。用限制性酶切片段长度多态分析 (RFLP)法对分离株NH 97进行鉴定 ,并同已知的西伯利亚立克次体等国际标准株进行比较。结果 从越原血蜱、金泽革蜱、微小牛蜱中扩增出SFGR特异的DNA片段 ,NH 97分离株的聚合酶链反应产物经PstI和RsaI酶切后发现它们的酶切图谱与西伯利亚立克次体 2 4 6国际标准株相同。从社鼠、黄胸鼠脏器中扩增出康氏立克次体DNA片段。由不同来源批次的越原血蜱中扩增出近缘于日本立克体DNA片段和相关序列提示 :可能是斑点热群立克次体新成员。结论 福建宁化林区除存在西伯利亚立克次体外 ,还可能存在康氏立克次体、日本立克次体等多种斑点热群立克次体的疫源地。
Objective To study the existence ofnatural foci of Spotted Fever in Ninghua, Fujian province. Methods Using DNA polymerase chain reaction and restriction endonuclease fragment length polymorphism analysis (PCR/RFLP)to detect spotted fever group rickettsiae (SFGR) in ticks and rodents. Results It was found that H. wellingtoni , H.yeni ,and Dermacentor auratus were infected with Rickettsia sibirica; the DNA fragments were cloned,the PCR products from isolated strain NH 97 were antigenically and genotypically identical to Rickettsia sibirica. Rattus flavipectus were found infected with R. conorii . One of the sequeuce analysis showed that the DNA sequence was different from other SFGR and close to R. japanic . Conclusion Natural foci of R. sibirica, R. sibrica, R. japanic and R.conorii are found in Ninghua, Fujian province of China.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2002年第2期106-108,共3页
Chinese Journal of Preventive Medicine
基金
福建省科技项目资助 (97 Z 5 8)
关键词
福建
宁化林区
鼠类
蜱类
斑点热群立克次体感染
检测
Rickettsia
Polymerase chain reaction
Polymorphism, restriction fragment length
