摘要
目的 研究锌对骨发育的影响。方法 用一次通气旋转装置 ,在缺锌的基础培养基和分别加入 4 5、70和 12 0 μmol/LZn2 + 的培养基中对 16d孕龄胎鼠前肢进行培养。结果 培养基中缺锌和在培养基中补充Zn2 + 至Zn2 + 浓度 12 0 μmol/L时 ,骨组织中骨钙素 (OC)和45Ca含量减少 ,碱性磷酸酶 (AKP)活性显著降低 ;当在培养基中补充Zn2 + 至Zn2 + 浓度 4 5、70 μmol/L时OC合成量、骨组织对钙的吸收及AKP活性显著增加。培养液中缺锌及锌浓度为 12 0 μmol/L时 ,X线显示 ,长骨长度和密度与其自身对照相比 ,长骨长度较短 ,骨密度降低 ;当培养液中Zn2 + 浓度分别为 4 5和 70 μmol/L时 ,与其自身对照相比 ,长骨长度及其骨密度有所增加。组织学分析显示 ,培养液中缺锌及锌浓度为 12 0 μmol/L时 ,镜下可见骨细胞死亡 ,基质丢失 ;当培养液中Zn2 + 浓度分别为 4 5和 70 μmol/L时 ,骨细胞增殖、分化活跃 ,类骨质的分泌与合成增加。结论 适量补锌能促进骨组织形成及发育 ,锌缺乏或过量时均可影响骨组织正常生长发育及代谢。
Objective This study was designed to explore the effects of zinc on bone development Methods Forelimbs of mice with 16 d gestation were cultured by a rotating device Results Contents of OC and 45 Ca and activities of AKP in the bone tissues cultured at zinc deficiency media and at media with 120 μmol/L of Zn 2+ decreased significantly Synthesis of OC, absorption of calcium and activities of AKP in the bone tissues cultures at media with 45 and 70 μmol/L of Zn 2+ increased significantly Radiograph of bone tissues showed that length of long bone cultured at zinc deficiency media and at media with 120 μmol/L of Zn 2+ shortened and their density reduced, and those cultured at media with 45和70 μmol/L of zinc increased, as compared with self control bone Histological analysis showed the death of bone cells and loss of stroma in the bone tissues cultured at media with 120 μmol/L of Zn 2+ , and active proliferation and differentiation of bone cells, and secretion and synthesis of osteoid increased in the bone tissues cultured at media with 45和70 μmol/L of zinc Conclusions Adequate supplementation of zinc could promote formation and development of bone tissues and deficiency or excess of zinc could alter their growth and development and normal metabolism
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2002年第2期97-99,F004,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助 (3 960 0 12 2 )
