摘要
利用SSR标记对分布在我国7个省的17个居群普通野生稻 OryzarufipogonGriff. 的种质资源进行研究,结果表明:17个居群普通野生稻的遗传多样性有着显著的差异,从UPGMA聚类结果可以看出普通野生稻的遗传多样性与其生态地理分布有显著的相关性,遗传多样性指数高的地区极有可能是栽培稻的起源中心;用筛选出的7对SSR引物对336份DNA样品进行扩增,得到60条特异条带,在分子水平上进一步证明普通野生稻的起源为两广地区;本研究也表明SSR是进行遗传资源多样性研究的一种切实有效的研究方法.
As cultivar rices progeny,Oryza rufipogon Griff. is a valuable wild germplasm.The genetic diversity of Oryza rufipogon Griff. was studied in 336 accessions of 17 chinese groups by SSR.The main results as followed:60 specific polymorphic patterns were observed after analyzing all the accessions by 7 SSR primers,there are significant differences among the 17 groups as well as the accessions in one group,The results of UPGMA shows conservation of Oryza rufipogon Griff.is stated on natural conservation.SSR marker is a very valuable molecular marker in Oryza rufipogon Griff. (na is 1.484 3,ne is 1.174 6,h is 0.115 0).
出处
《西北植物学报》
CAS
CSCD
2003年第10期1750-1754,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家科技部863项目(2001AA241-37)
