摘要
通过DNA染色剂 染色质配合过程的荧光寿命、精确量热和荧光能量转换的测定 ,研究了正常染色质 (NC)和肿瘤染色质 (TC)的结构。结果表明 ,NC中溴化乙锭插入DNA的成分比TC增加了 4 86 % ;溴化乙锭与TC中DNA的配合焓为 - 15 38kJ/mol,NC中DNA的配合焓为 - 13 2 5kJ/mol;TC中丹磺酰氯和吖啶橙之间的荧光能量转换效率比NC中的低 ,而平均供体 受体距离比NC的大 ,说明TC刚性结构较少、与溴化乙锭结合的可能性较大及基因活性较强。
The structures of rat liver chrotin and its walker tumoral chromatin have been studied by fluorescence lifetime, precision calorimetric determinations and energy transfer measurement. The results show that the component with ethidium bromide inserting DNA in tumoral chromatin(TC) increases by 4.86% than that in normal chromatin(NC). The formation enthalpy of the complex from ethidium bromide and DNA in TC is -15.38 kJ/mol and -13.25 kJ/mol for NC. The energy transfer efficiency between dansyl chloride and acridine orange in labeled TC is lower than that in labeled NC. The mean donor-acceptor distance is longer than that in labeled NC. These results denote that TC has a less rigid structure and higher genic activity, and can more easily form complex with ethidium bromide.
出处
《应用化学》
CAS
CSCD
北大核心
2003年第12期1135-1138,共4页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金 ( 2 970 10 0 5 )
中国科学院基础局重大项目 (KJ95 1 A1 5 0 4 0 2 )资助项目
关键词
量热学
染色质
能量转换
荧光寿命
chromatin,calorimetry,energy transfer,fluorescence lifetime
