摘要
用辣根过氧化物酶标记的ConA、WGA和RCA Ⅰ为探针,研究了小鼠发育期间角膜内糖残基的分布和变化。Man残基主要分布在角膜基质和内皮;SA残基主要存在于角膜上皮;Gal残基在角膜上皮和基质中都有分布。Man残基在出生前后的小鼠角膜基质中朝内皮方向呈现递增的梯度。SA和Gal残基随角膜发育最后在成体角膜上皮的外表而密集。胎龄13天是小鼠角膜成纤维细胞合成复合糖的起始时间。
Using ConA-HRP and RCAI-HRP as probes, the distribution and changes of glycoresidues in mouse cornea were studied during pre- and postnatal development. Mannose residues were distributed mainly in stroma and endothelium sialic acid residues in epithelium and galactose residues in both epithelium and stroma. Mannose residues in stroma showed an increased density toward endothelium before and after birth. Sialic acid and galactose residues were concentrated gradually at the corneal epithelial surface in accompanied with the development of cornea. The embryonic day 13 was the starting day to synthesize glycoconjugates from fibroblasts of mouse cornea.
出处
《实验生物学报》
CSCD
1992年第4期339-347,共9页
Acta Biologiae Experimentalis Sinica
基金
甘肃省自然科学基金
关键词
角膜
发育
凝集素
受体
Cornea. Development. Lectin. Glycoconjugate. Histochemistry.
