摘要
观察了菠菜叶绿体类囊体膜与残缺膜的表面结构及鼠肝线粒体嵴膜小囊在制备过程中的生成过程,证明了嵴膜小囊可有两种,F_1在膜外侧或膜内侧,它们都可与残缺膜组成镶嵌膜,进行光下磷酸化功能。而F_1在膜外侧的嵴膜小囊与残缺膜的嵌合膜活力更高。
The structure of the fusion membranes from the deficient thylakoid membranes of spinach chloroplasts with the crista vesicles of the rat liver mitochondria was studied by electron microscopic examination which the hanging drop and negative staining, and thin section methods were used.
We can see that many phosphorylation coupling factors (CF_1) are bound on the surface of the thylakcid membranes (Plate Ⅰ,Figure 1). When the CF_1 were removed by EDTA solution, the phosphorylation activity was lost to form the deficient thylakoid membranes which the surface is very smoothly (Plete Ⅰ, Figure 2).
The production procedure of the crista vesicles from mitochondria during preparation was observed (Plate Ⅰ, Figure 3 A-C). Plate Ⅰ,Figure 3 B indicated that the crista membranes just break out the envelope of mitochondria, then the membranes were broken up to the fragments in hyrotonic solution (Plate Ⅰ,Figure 3 C-1), and then were swollen to crista vesicles (Plate Ⅰ, Figure 3 C-2).
Crista vesicles can be separated to two bands by SDG centrifugation (Fig. 1-A), in the upper band, the phosphorylation coupling factors (F_1) are on the out-side of the crista vesicles (Plate Ⅰ, Figure 4), and in the lower band, the F_1 are on the in-side of the crista vesicles (Plate Ⅰ,Figure 5).
Both of the two kinds of crista vesicles can combine with the deficient thylakoid membranes to form fusion system which reconstituted the phosphorylation activity in light (Table 1). The fusion mode is that the crista vesicles are inlaid to the apparent sight (Plate Ⅰ, Figure 6) and by the cross section (Plate Ⅰ, Figure 7) of the fusion membranes.
出处
《实验生物学报》
CSCD
1992年第1期1-7,共7页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
关键词
残缺类囊体膜
嵴膜小囊
融合膜
Deficient thylakoid membranes. Crista vesicles of mitochondria. Fusion membranes.
