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绵羊口腔真角化上皮和类角化上皮中膜包颗粒的观察 Ⅰ.不同固定剂对膜包颗粒超微结构的影响

OBSERVATIONS OF MEMBRANE-COATING GRANULES (MCG) IN ORTHOKERATINIZED AND PARAKERATINIZED SHEEP ORAL EPITHELIA
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摘要 用三种固定方法固定绵羊口腔上皮。根据对不同固定剂的反应,区分出两种类型膜包颗粒。硬腭上皮含Ⅰ型颗粒,其颗粒的内部结构随固定方法不同而变化。齿板和丝状乳头上皮含Ⅱ型颗粒。不同固定剂对此型颗粒的超微结构不引起明显变化。两型颗粒均存在于软腭上皮中。根据不同固定剂对膜包颐粒的影响,Ⅰ型颗粒可能富含糖蛋白和脂蛋白,Ⅱ型颗粒存在含游离氨基的磷脂类。在硬腭和软腭上皮中,伴随膜包颗粒数量减少,朝向上皮游离面的细胞膜表面覆盖了一层电子致密物质。此物质对不同固定剂的反应与Ⅰ型颗粒的内含物相同。这层表面物质可能与上皮的渗透屏障作用有关。 Two types of MCG were divided accoring to their reaction to different fixation schedules. Type Ⅰ MCG occurred in palatine rugae. They showed an electron dense periphery and translucent center or a dense core with translucent zone between the dense core and dense periphery when tissues were non-osmicatcd. After osmication most of type Ⅰ MCG showed mesh-like content and a few lamellar content. Type Ⅱ MCG were found in dental pad filiform papillae. They did not have distinct change with different fixation schedules and showed lamellar or mesh-like content, or homogeneous electron dense content. Both types of MCG appeared in soft palate. According to the results, it is suggested that type Ⅰ MCG contains glycoproteins and lipoproteins and type Ⅱ MCG have the phospholipids conaining free amino groups. In palatine rugae and soft palate, following discharge of MCG, a layer of substance which showed the same appearance as the content of MCG coated on the superficial cell surface in intercellular space. The membrane coating may provide permeability barrier to the epithelium.
作者 王石平
出处 《解剖学杂志》 CAS CSCD 北大核心 1989年第3期185-189,共5页 Chinese Journal of Anatomy
关键词 膜包颗粒 绵羊 口腔上皮 Membrane-coating granules, Sheep, oral epithelium.
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