摘要
本文应用透射电镜研究 MMC-1类巨噬细胞对调理化了的鸡红细胞的吞噬现象。将兔抗鸡红细胞抗血清与鸡红细胞共同孵育后,可见10至10余个调理化的红细胞围绕在 MMC-1细胞周围,形成玫瑰花环。在此两细胞的接触点,显现电子致密带;其后鸡红细胞出现多个小突起,并被吞入 MMC-1细胞中。继之 MMC-1细胞的丝足呈衣领状,包绕被吞入的红细胞;当红细胞大部被吞入时,则呈哑铃状。被吞入的红细胞外形不规则,胞质电子密度逐渐降低,另一方面却出现了逐渐扩大的高电子致密环。这证实形成玫瑰花环的红细胞已被 MMC-1细胞所吞噬,并在 MMC-1细胞内退变,表明 MMC-1细胞具有 F_c 受体并有吞噬功能。在 MMC-1细胞中有大量线粒体,可能与吞噬过程所需能量有关。另外还见到被吞噬的细胞,由巨噬细胞排出的现象。根据这种细胞的来源与功能特点认为,命名此 MMC-1细胞系为 MMC-1类巨噬细胞系是恰当的。
The phagocytosis of opsonic chicken erythrocytes which were engulfed by
MMC-1 cells was studied by means of transmission electron microscopy.After incu-
bating chicken erythrocytes with the serum of rabbit-anti chicken antibody.MMC-1
cells were surrounded by 1 to more than 10 opsonized erythrocytes.The EA rosettes
were formed.At first,opsonized erythrocytes attached to the MMC-1 cells in some
points.At the site of interaction of opsonized erythrocytes with MMC-1 cells the
electron dense bands which were the specialization of the MMC-1 cell plasma mem-
brane appeared.Then many projections of the erythrocytes were engulfed into
MMC-1 cells.When part of the erythrocyte was engulfed by macrophage,a collar-
like structure arround the erythrocyte could be seen.Partially engulfed erythrocytes
looked like a dumbbell.The engulfed erythrocytes were irregular in shape.The
density of engulfed erythrocytes became faint gradually.The round ring with high
electron density appeared and enlarged in erythrocytes.It was shown that the opso-
nized erythrocytes which formed EA rosettes were engulfed by MMC-1 phagocyte.
After engulfing many erythrocytes,MMC-1 cells might degenerated or be engulfed
by other MMC-1 cells.It was proved that Fc receptors presented on MMC-1 cells.
Many mitochondria were easily found in MMC-1 cells,they were responsible for
energy supplies.Extruding of engulfed nuclei from macrophage could be seen too.
It was shown that MMC-1 cells had the characteristics of macrophage and it was
established by cell fusion of phagocyte and thymoma cell.
出处
《解剖学报》
CAS
CSCD
北大核心
1989年第3期269-272,共4页
Acta Anatomica Sinica
关键词
巨噬细胞
吞噬作用
透射电镜
Phagocytosis
MMC-1 macrophage-like cell line
Opsonized chicken erythrocyte
Fc receptor
Transmission electron microscope
