摘要
乙型肝炎病毒(HBV)前S_2蛋白(Pre-S_2蛋白)作为HBV感染的新标志,在流行病学、临床、预后判断(成人)及免疫病理的意义正在研究中。其在小儿乙型肝炎(乙肝)时临床上价值的研究未见报道。本文以单克隆抗体,酶联免疫法(Enzyme-linked immunoassay ELISA)检测经肝活检证实的49例乙肝患儿血清标本151份。研究其与临床、病理及血清和肝脏乙肝病毒感染标记(Hepatitis B virus marker,HBVM)的相互关系。
49 children (m31, f18 ) with pathologically confirmed hepatitis B hospitalized from July 1986 to July 1987 were enrolled in this study. 151blood samples from 49 children were investigated for their positivities of Pre-S_2 using enzyme-linked immunoassay ( ELISA ) with monoclonal antibody (McAb),and hepatitis B virus markers using avidin-biotin complex (ABC) and enzyme immunoassay (EIA). Hepatitis B virus markers were also investigated on the paraffin sections of 49 liver biopsy specimens using avidinbiotin complex(ABC). The results indicated that the detection of Pre S_2 was correlated well with plasma HBsAg titre (p<0.01), and the positivities of histological HBsAg (p<0.01).The serum Pre-S_2 positivities in patients (56.88%) with HBeAg in serum were significantly higher than that in patients (28.26%) without HBeAg in serum (p<0.001), but the serum Pre-S_2 positivities in patients with histological HBeAg(61.54%)were not significant to that in patients without histological HBeAg (78.57%).Pre-S_2 positivities for patients with histological HBcAg were significantly higher than that of patients without histological HBcAg (91.67 vs 50.00% p<0.01 ) indicating that Pre-S_2 could reflect the presence of histological HBcAg.The average detectable rate for serum Pre-S_2 in this group was 50.33%. The average rate of conversion to the negtivities of Pre-S_2 was 16.33% in 3～6 months. The persistent positive rate of serum Pre-S_2 in this group was 48.98%. We noticed that the persistent positive rate for male patients was significantly higher than that for female patients (61.29% vs 27.78%, p<0.05).The results also indicated that the detectable rate of Pre-S_2 in serum of the children was significantly lower than that of the adults(50.33% vs 73.43%. p<0.05).
出处
《解放军预防医学杂志》
CAS
北大核心
1989年第4期366-371,共6页
Journal of Preventive Medicine of Chinese People's Liberation Army
