摘要
以烟草myb1中MYB保守区域编码序列的两个相对特异性序列为引物 ,以表现过敏性反应(hypersensitiveresponse,HR)的番茄苗中提取的RNA为模板 ,通过RT -PCR获取长度为 2 15bp的myb基因片段LeMYB1和LeMYB2 (GenBank核酸序列数据库登录号 :AY1312 30和AY1312 31)。两者只有第 6 3位碱基不同 ,前者序列为A ,而后者为G。Northern杂交分析表明 ,LeMYB相关基因表达时序与番茄苗HR的产生和发展呈密切正相关 ,因而可能在Cf
Two 215 bp myb gene fragments,LeMYB1 and LeMYB2 w it h GenBank accession No.AY131230 and AY131231 respectively,were cloned through RT -PCR.The pri mers were designed in accordance with tobacco myb1 at the DNA-binding domai n conserved in MYB,and the template was the RNA isolated from tomato seedlings showing hypersensitive responses(HR).Only nucleotide 63 was different between the two fragments,of which LeMYB1 was A and LeMYB2 was G.Three nucleo tides within the primers for cloning of LeMYB1 were different between LeMYB1 and counterpart of THM18,whereas 90 7% nucleotide sequence of LeMYB1 was identical to corresponding parts of tobacco myb1 .Northern assay revealed that expression of LeMYB1-related genes were ind uced upon occurrence of hyperse nsitive necrosis in tomato seedlings and enhanced henceforth.Their coincident ex pression with HR development in tomato indicated that LeMYB1-related genes might be involved in regulation of HR and disease resistance conferred by tomato fungal resistance gene Cf-9.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第5期589-591,共3页
Acta Horticulturae Sinica
基金
国家自然科学基金资助项目 (3 0 0 70 492 )
