摘要
目的 探讨血清转化生长因子-β_1(TGF-β_1)的动态变化与脑梗死患者炎症损害的机理、脑梗死灶体积和临床预后的相互关系。方法 采用双抗体夹心酶联免疫吸附法(ELISA)规定29例脑梗死患者发病后1、5、10d的血消TGF-β_1水平,并与健康体检者25例对照。结果 脑梗死患者血清TGC-β_1水平在发病第1d(24.57±7.4)mg/L明显低于对照组(46.22±9.3)mg/L,第5d(62.82±9.6)mg/L明显高于对照组(45.98±9.0)mg/L,并具统计学差异性(P<0.05,P<0.01),第10d两组无明显差异(P>0.05),且脑梗死组3个时段相互比较也存在明显差异(P<0.01);脑死梗灶体积≤6cm^3为13例,>6cm^3为16例,血清TGF-β_1低谷值与梗死灶大小无关(P>0.05),但高峰值与梗死灶大小有关(P<0.05);血清TGF-β_1与初期病情轻重无关(P>0.05),和3个月时神经功能缺损程度评分呈负相关(P<0.05)。结论 血清TGF-β_1参与了脑梗死发生发展的病理生理过程,其水平测定对疾病的转归、预后的判断以及外源性TGF-β_1临床应用,提示了具有积极意义的思维方向。
Objective: To investigate the transforming growth factor-β1 (TGF-β1) development and relation among the inflammation damage mechanism, volume, clinical prognosis in cerebral infraction (CI) patient. Methods: Determine the serum TGF-β1 level of 29 CI patients with the first, the 5th, and the 10th day by ELISA. Results: Among the CI patients the TGF-β1, level of the 1st day(24. 57 ±7. 4 mg/L) lower than the contrast group(46. 22±9. 3 mg/L), of the 5th day (62. 82±9. 6 mg/L) higher than the contrast group (45. 98±9. 0 mg/L). There is a atatistical difference ( P < 0. 05 or P < 0. 01) in the 1st and 5th day without in the 10th day ( P > 0. 05), The difference is obvious among the 3 time groups ( P < 0. 01 ) . The volume(V) in 12 CI patients V≤6 ml, in 16 patients V > 6 ml. The lowest value of TGF-β1 has no relation with the lesion size( P > 0. 05) . The highest value relates with the lesion size ( P < 0. 05) . There is no relation between the CI progress and TGF-β1 level ( P > 0. 05), but we discover there is negative relation with the degree of neural function defection 3 months later ( P < 0. 05). Conclusion: TGF-β1 takes part in the pathophysiological process of CI. If we had offered extragenic TGF-β1 in early stage, the condition would be improved. The level determination may be helpful for the disease progress, the estimate prognosis, and externality TGF-β1 treatment.
出处
《循证医学》
CSCD
2002年第1期27-30,共4页
The Journal of Evidence-Based Medicine
