摘要
目的 现场评价自制的恶性疟Ｄｉｐｓｔｉｃｋ－免疫胶体金检测试纸条和商品疟疾ＩＤＣＴ测试卡的敏感性和特异性及稳定性。方法 以常规镜检法作为标准，用Ｄｉｐｓｔｉｃｋ条和ＩＣＴ卡对镜检确认的疟疾和非疟发热病人血样平行检验。结果 Ｄｉｐｓｔｉｃｋ条恶性疟阳性的符合率为９５％，特异性为１００％，３０℃以上活性保持时间为４５天左右；ＩＣＴ卡的恶性疟（含混合感染）阳性符合率为１００％，单纯间日疟符合率为９７％，特异性为１００％，不能区分混分感染，稳定性超过Ｄｉｐｓｔｉｃｋ条。结论Ｄｉｐｓｔｉｃｋ条仍需改进完善。ＩＣＴ卡敏感特异、简便快速，是替代传统镜检较好的一种方法。
Objective These assays were to evaluate the specificity、sensitivity and stablity of the dipsticks that were made in our laboratory to diagnose the plasmodium falciparun infection. Methods Two specialists inde- pendently tested the samples with microscope thick film and thin film at the same time, distinguished the P. falci- parum or P. vivax infection, and the eventually confirmed result was regarded as gold standard. 50 malaria -in- fected samples and 100 nonmalaria-infected samples from fever patients at the same local were all tested with dip- stick and ICT card. Results the specificity of two methods were 100%, the sensitivity of dipstick to p. falcipa- rum was 95%,the sensitivity of ICT top. falciparum was 100%,the sensitivity of ICT top. vivax was 97%. ICT card was more stable than dipstick, the validity of the dipsticks could only maintain about 45 days at tempera- ture over 30℃ . Conclusion ICT card nearly substitute the traditional microscopy method, altough it is not very satisfactory for the diagnosis of p. vivax. Dipstick requires more improvements in its stablity and sensitivity.
出处
《中国热带医学》
CAS
2001年第3期199-200,共2页
China Tropical Medicine
基金
广东省卫生厅青年自然科学基金资助(98-332)
