摘要
目的 :研究肺炎链球菌呼吸道感染分离株的耐药性及其传播情况。方法 :肺炎链球菌分离株进行药物敏感试验和血清学分型 ,并结合BOXPCR、脉冲场电泳 (pulse-fieldgelelectrophoresis,PFGE)和青霉素结合蛋白基因 (penicillinbindingpro teingene,pbp)指纹等分子生物学方法分析菌株间亲缘关系。 结果 :呼吸道感染分离株 1 2 8株中有青霉素低度耐药株 (peni cillinintermediatedStreptococcuspneumoniae,PISP) 1 0株 ,未发现青霉素高度耐药株 (penicillinresistantStreptococcuspneumoni ae,PRSP)。 1 1 1株存活菌株分属 2 5种血清型 ,主要为 2 3F、3、1 9F、1 4、9V、6A、6B等。 9株存活PISP中有 6种PFGE谱型 ,2株PFGE谱型相同菌株的耐药谱和血清型相仿 ;2株不同PFGE谱型菌株pbp指纹相似 ,对青霉素和头孢曲松敏感性相似 ;上述呼吸道感染分离PISP中未发现与世界主要流行耐药克隆代表株DNA指纹或 pbp基因相似菌株。 结论 :上述肺炎链球菌呼吸道感染分离株青霉素耐药率尚低 ,存在青霉素耐药克隆和基因的传播 ,其中尚未发现世界主要流行耐药克隆。
Purpose:To investigate the resistance and spread of Streptococcus pneumoniae isolated from patients with respiratory tract infections. Methods:The homology among strains was determined by antimicrobial resistance pattern, serotyping, BOX PCR, PFGE and pbp fingerprint. Results:Among 128 isolates,118 were penicillin sensitive strains(PSSP),10 strains were penicillin intermediate Streptococcus pneumoniae (PISP), no penicillin resistant Streptococcus pneumoniae (PRSP). One hundred and eleven surviving strains belonged to 25 serotypes, the main serotypes being 23F, 3, 19F, 14, 9V, 6A, 6B, et al. Six PFGE patterns were found in 9 surviving PISP, and strains of same PFGE pattern possessed similar resistance patterns and belonged to same serotypes. Two strains of different PFGE patterns possessed similar pbp fingerprints and susceptiblily to penicillin and ceftriaxone. PFGE patterns and pbp fingerprints of these clinical isolates were different from those of the worldwide prevalent resistant clones. Conclusions:Penicillin resistant rate of these S. pneumoniae strains was still low. Among these strains, the spread of penicillin resistant clone and resistant gene could exist, no worldwide prevalent resistant clone was found.
出处
《中国抗感染化疗杂志》
2001年第1期13-16,共4页
Chinese Journal of Infection and Chemotherapy
