摘要
通过对低渗溶血过程、荧光淬灭效应及阴离子跨膜通透性的研究,探讨了苯肼对红细胞膜结构和功能的影响。苯肼浓度0.01mM时,低渗溶血的K_1快过程开始变慢,表明膜脂质流动性的降低。苯肼浓度增至0.1mM后,膜和变性血红蛋白的结合大为增强,这种膜结构的变化提高了阴离子的跨膜通透性。
The membrane-Hb interaction was modified by treating intact erythrocytes with phenylhydrazine. The effect of the modification on structure and function of erythrocyte membrane was studied by measuring osmotic hemolytic process, fluorescent quenching, and anion transport. The fast process (K1) of osmotic hemolysis was affected at phenylhydrazine concentration >0.01 mM. This means that the membrane fluidity is decreased. The binding of Hb to the membrane was increased rapidly at phenylhydnazine concentration > 0.1 mM. Furthermore, we observed that the anion transport across erythrocyte membrane was increased due to change of membrane structure.
出处
《生物物理学报》
CAS
CSCD
北大核心
1992年第3期507-511,共5页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
红细胞膜
苯肼
结构
功能
Erythrocyte membranes, phenylhydrazine, the rate of osmotic hemolysis, fluorescent quenching, anion transport.
