摘要
用三聚氯氰活化单甲氧基聚乙二醇,得到了双链聚乙二醇(PEGm_2).以PEGm_2修饰牛血Cu,Zn-SOD,得到了氨基修饰率为30％,残余活力为80％的纯PEGm_2-SOD.修饰酶的荧光光谱及圆二色谱均有改变;盐酸胍变性及胃蛋白酶水解实验结果表明,修饰酶的稳定性高于天然酶;免疫学实验表明,与天然酶比较,修饰酶的免疫原性及抗原抗体反应性大为降低.
Bovine Cu, Zn-SOD was modified by 2,4-bis (O-methoxypolyethylene glycol)-6-Chloro-S-triazine (activated PEGm_2) in different rates between amino groups of SOD and PEGm_2 The purified PEGm_2-SOD that remained 80％ residual activity with 30％ modified amino groups was obtained. The fluorescence spectrum and circular dichroism of modified SOD were changed; the results of denaturation by GuHCl and of hydrolysis by pepsin showed that PEGm_2-SOD had higher stability than SOD; the results of immunology showed that the immunogenicity and antigenicity of PEGm_2-SOD decreased greatly.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1992年第6期444-448,共5页
Progress In Biochemistry and Biophysics
