摘要
应用PCR及寡核苷酸探针杂交方法研究了50例人原发性肺癌石蜡切片标本 中Ki-ras 12(Val),Ki-ras 13(Cys)点突变,发现13例为点突变阳性,突变率为26％,其中3例同时兼有二种点突变(Val/Cys);Ki-ras 12位的点突变率(20％)比Ki-ras 13位的点突变率(12％)高。同时还发现Ki-ras基因的点突变存在于腺癌、鳞癌、小细胞肺癌以及各种分化程度的肺癌中。
The frequency of oncogene Ki-ras12 (Val), Ki-ras13 (Cys) mutation was studied in paraffin embedded sections obtained from 50 human lung cancer by in vitro amplification of target sequences via polymerase chain reaction (PCR)and selective oligonucleotide hybridization. 13 samples (26%) contained Ki-ras mutation. 3 carcinomas exhibited 2 distint Ki-ras mutation. The Ki-ras mutation was present in adenocarcinoma, squamous cell carcinoma, adenosquamous carcinoma, well differentiated, moderately differentiated, poorly differentiated human lung cancers.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1992年第4期277-281,共5页
Progress In Biochemistry and Biophysics
基金
国家"七.五"攻关资助项目
