摘要
本文研究了一种用于测定发酵液谷氨酸含量的介质电流型谷氨酸电极。这种电极是由谷氨酸氧化酶同以二茂铁为介质的化学修饰电极复合构成。研究了各种因素对此电极响应的影响和电极本身的性能与实测验证,结果表明本电极在搅拌反应室(CSR)系统中响应的最适pH为6.8,最适温度为30℃,使用寿命为6天,电极专一性较好。在流通注射分析(FIA)系统中响应的最适pH为7.0,最适温度为27℃,测量范围为0.0125—0.125mol/L(注射(20μ1),精度(CV)为1.3%,响应时间为1min,校正曲线相关系数r=0.998,使用寿命为8天,经实际测量发酵液中谷氨酸含量,与瓦氏呼吸仪对照,相对误差为±6%。
The study on a new glutamate biosesor, which was mainly composed of glutamate oxidase, ferrocene, graphite and platinum disc was presented. Various factors which influence the response of the biosensor to glutamate and the performance of the electrode were studied. The results show that the optimum pH and temperature for the electrode in CSR were 6.8 and 30℃ respectively, then in FIA 7.0 and 27℃ respectively. Among 25 substrates tested, no apparent response were observed for sugars, organic acids and amino acids, except arginine and alanine. Good linear relationship was obtained within the range of 2.5—25μl(0.1mol/L)glutamate. The correlation coefficient of the calibration ourve, r=0.998, the response time was 1 min, and the useful life of the sensor was about one week. Satisfactory results were obtained when the biosensor was applied to the determination of glutamate in fermentation process.
出处
《生物工程学报》
CAS
CSCD
北大核心
1992年第4期394-400,共7页
Chinese Journal of Biotechnology
关键词
谷氨酸
测定
生物传感器
二茂铁
Glutamate determination
biosensor
glutamate oxidase
chemically modified electrode
enzyme electrode
