摘要
我们克隆了含高粱叶绿体psaA基因的6.7kb EcoRI酶切片段,进行了限制酶图谱分析和核苷酸序列结构测定,所测核苷酸序列总长为3080bp,其中高粱叶绿体psaA基因序列为2253bp,编码一个含750个氨基酸残基的蛋白质,其分子量为83000Da。高粱psaA基因的核苷酸序列与玉米、水稻、菠菜的同源性分别为99.4%、96.3%和89.4%;推导出的氨基酸序列的同源性分别为99.3%、98.0%和95.7%。C_4植物与C_3植物比较,由于P_(700)脱辅基蛋白的第493位氨基酸性质的不同(Gly→Arg,Ser),而导致了它们的多肽在疏水性图谱上的差异。
The EcoRI-6.7 kb fragment containing the sorghum chloroplast psaA gene was cloned. The restriction endonuclease map and the nucleotide sequence were determined.The length of the determined nucleotide sequence was 3080 bp, of which the psaA coding sequence was 2253 bp, which encoded a 750 amino acid polypeptide with a predicted molecular weight of 83 000. The homologies of this psaA gene with maize, rice and spinach were 99.4%, 96.3% and 89.4%, respectively, and the homologies of the putative polypeptide sequences were 99.3%, 98.0%, 95.7% respectively. We found that a discrepancy in the plot of hydropathy distribution of P_(700) apoprotein existed between C, and C_3 plants due to a change occurred at the 493th amino acid (C_4 plant:Gly-C_3 plants:Arg or Ser).
出处
《生物工程学报》
CAS
CSCD
北大核心
1992年第3期207-212,共6页
Chinese Journal of Biotechnology
基金
国家高科技“863-101-09-37”基金
